Janelia hosted a series of nine 4D Cellular Physiology workshops that were aimed at thinking about questions at this emerging interface between cell biology and physiology. In this series, rather focusing on past results, the speakers addressed important questions for the future and ways to overcome barriers. The workshops introduced us to wonderful colleagues around the world. We encourage you to watch a few of our featured workshop talks (10 min each) to get a feel for the types of problems that could be addressed in a 4D Cellular Physiology research program. Visit the Janelia YouTube Channel for full recordings of all past workshops.